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Isolation, culture, and functional analysis of hepatocytes from mice with fatty liver disease
We present a protocol for isolating hepatocytes from mice with established non-alcoholic fatty liver disease. This protocol consists of liver perfusion using a peristaltic pump, followed by a modified 25% and 90% Percoll gradient centrifugation protocol to capture lipid-laden hepatocytes that are us...
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| Pubblicato in: | STAR Protoc |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757664/ https://ncbi.nlm.nih.gov/pubmed/33377114 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100222 |
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