載入...
Isolation, culture, and functional analysis of hepatocytes from mice with fatty liver disease
We present a protocol for isolating hepatocytes from mice with established non-alcoholic fatty liver disease. This protocol consists of liver perfusion using a peristaltic pump, followed by a modified 25% and 90% Percoll gradient centrifugation protocol to capture lipid-laden hepatocytes that are us...
Na minha lista:
| 發表在: | STAR Protoc |
|---|---|
| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2020
|
| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757664/ https://ncbi.nlm.nih.gov/pubmed/33377114 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100222 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|