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Protocol for Monitoring DNA-Triggered cGAS/STING Signaling in Mammalian Cells and Mice
Innate immunity is the first layer of defense against infection in mammals and is tightly regulated. We monitored cGAS/STING signaling upon ISD90 or 2′,3′-cGAMP stimulation in EA.hy926 cells by western blotting, RT-PCR, and ELISA analyses to reveal signaling activation and IFNβ production. In additi...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757363/ https://ncbi.nlm.nih.gov/pubmed/33377065 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100171 |
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