Protocol for Monitoring DNA-Triggered cGAS/STING Signaling in Mammalian Cells and Mice

Innate immunity is the first layer of defense against infection in mammals and is tightly regulated. We monitored cGAS/STING signaling upon ISD90 or 2′,3′-cGAMP stimulation in EA.hy926 cells by western blotting, RT-PCR, and ELISA analyses to reveal signaling activation and IFNβ production. In additi...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Wang, Ying, Ma, Zhe, Damania, Blossom, Liu, Pengda
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757363/
https://ncbi.nlm.nih.gov/pubmed/33377065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100171
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