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Determining Alternative Protein Isoform Expression Using RNA Sequencing and Mass Spectrometry
Alternative splicing greatly expands the coding capacity of the human genome, but how many alternative transcripts are translated as proteins or carry functional importance remains unknown and awaits experimental verification. Here, we describe a protocol that combines transcriptomics (RNA-seq) and...
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| Publicado en: | STAR Protoc |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757315/ https://ncbi.nlm.nih.gov/pubmed/33377032 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100138 |
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