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Determining Alternative Protein Isoform Expression Using RNA Sequencing and Mass Spectrometry

Alternative splicing greatly expands the coding capacity of the human genome, but how many alternative transcripts are translated as proteins or carry functional importance remains unknown and awaits experimental verification. Here, we describe a protocol that combines transcriptomics (RNA-seq) and...

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Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Han, Yu, Wright, Julianna M., Lau, Edward, Lam, Maggie Pui Yu
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2020
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757315/
https://ncbi.nlm.nih.gov/pubmed/33377032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100138
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