Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids

Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Heinz, Maria C., Oost, Koen C., Snippert, Hugo J.G.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757012/
https://ncbi.nlm.nih.gov/pubmed/33377020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100126
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