CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells

CRISPR-Cas9 genome engineering can be used to functionally investigate the complex mechanisms of immune system regulation. Decades of work have aimed to genetically reprogram innate immunity, but current approaches are inefficient or nonspecific, limiting their use. Here, we detail an optimized stra...

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Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Hildreth, Andrew D., Riggan, Luke, O’Sullivan, Timothy E.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2020
Ämnen:
Protocol
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7756923/
https://ncbi.nlm.nih.gov/pubmed/33377009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100113
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