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CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells

CRISPR-Cas9 genome engineering can be used to functionally investigate the complex mechanisms of immune system regulation. Decades of work have aimed to genetically reprogram innate immunity, but current approaches are inefficient or nonspecific, limiting their use. Here, we detail an optimized stra...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:STAR Protoc
Päätekijät: Hildreth, Andrew D., Riggan, Luke, O’Sullivan, Timothy E.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2020
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7756923/
https://ncbi.nlm.nih.gov/pubmed/33377009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100113
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