Quantitative Imaging of MS2-Tagged hTR in Cajal Bodies: Photobleaching and Photoactivation

Advances in imaging technologies, gene editing, and fluorescent molecule development have made real-time imaging of nucleic acids practical. Here, we detail methods for imaging the human telomerase RNA template, hTR via the use of three inserted MS2 stem loops and cognate MS2 coat protein (MCP) tagg...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Smith, Michael, Querido, Emmanuelle, Chartrand, Pascal, Sfeir, Agnel
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7756913/
https://ncbi.nlm.nih.gov/pubmed/33377008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100112
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