Quantitative Imaging of MS2-Tagged hTR in Cajal Bodies: Photobleaching and Photoactivation

Advances in imaging technologies, gene editing, and fluorescent molecule development have made real-time imaging of nucleic acids practical. Here, we detail methods for imaging the human telomerase RNA template, hTR via the use of three inserted MS2 stem loops and cognate MS2 coat protein (MCP) tagg...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:STAR Protoc
Egile Nagusiak: Smith, Michael, Querido, Emmanuelle, Chartrand, Pascal, Sfeir, Agnel
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Elsevier 2020
Gaiak:
Protocol
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7756913/
https://ncbi.nlm.nih.gov/pubmed/33377008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100112
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