Quantitative Imaging of MS2-Tagged hTR in Cajal Bodies: Photobleaching and Photoactivation

Advances in imaging technologies, gene editing, and fluorescent molecule development have made real-time imaging of nucleic acids practical. Here, we detail methods for imaging the human telomerase RNA template, hTR via the use of three inserted MS2 stem loops and cognate MS2 coat protein (MCP) tagg...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Gepubliceerd in:STAR Protoc
Hoofdauteurs: Smith, Michael, Querido, Emmanuelle, Chartrand, Pascal, Sfeir, Agnel
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2020
Onderwerpen:
Protocol
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7756913/
https://ncbi.nlm.nih.gov/pubmed/33377008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100112
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items