CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting. Fluorescent proteins allow stoichiometric labeling of cellular proteins; however, most methods either lead...

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Publicado no:iScience
Main Authors: Baldering, Tim N., Karathanasis, Christos, Harwardt, Marie-Lena I.E., Freund, Petra, Meurer, Matthias, Rahm, Johanna V., Knop, Michael, Dietz, Marina S., Heilemann, Mike
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7753144/
https://ncbi.nlm.nih.gov/pubmed/33364584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2020.101895
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