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Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency

BACKGROUND: Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or modify target DNA cassettes. One important metabolic engineering strategy is to l...

詳細記述

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書誌詳細
出版年:Microb Cell Fact
主要な著者: Xie, Qianwen, Li, Siwei, Zhao, Dongdong, Ye, Lijun, Li, Qingyan, Zhang, Xueli, Zhu, Li, Bi, Changhao
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2020
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7737257/
https://ncbi.nlm.nih.gov/pubmed/33317552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01496-w
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