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Exploiting dCas9 fusion proteins for dynamic assembly of synthetic metabolons

Here we reported a new strategy to construct synthetic metabolons using dCas9-guided assembly. Three orthogonal dCas9 proteins were exploited to guide the independent and site-specific assembly of their fusion partners onto a single DNA scaffold. This new platform was applied towards the constructio...

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Publicat a:Chem Commun (Camb)
Autors principals: Berckman, Emily A., Chen, Wilfred
Format: Artigo
Idioma:Inglês
Publicat: 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7725109/
https://ncbi.nlm.nih.gov/pubmed/31210215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9cc04002a
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