Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing

The enzymatic production of prebiotic fructo-oligosaccharides (FOS) from sucrose involves fructosyltransferases (FFTs) and invertases, both of which catalyze forward (transferase) and reverse (hydrolysis) reactions. FOS yields can therefore be increased by favoring the forward reaction. We investiga...

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Bibliografische gegevens
Gepubliceerd in:Front Bioeng Biotechnol
Hoofdauteurs: Burghardt, Jan Philipp, Fan, Rong, Baas, Markus, Eckhardt, Dustin, Gerlach, Doreen, Czermak, Peter
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Frontiers Media S.A. 2020
Onderwerpen:
Bioengineering and Biotechnology
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7724039/
https://ncbi.nlm.nih.gov/pubmed/33324627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.607507
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