Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing

The enzymatic production of prebiotic fructo-oligosaccharides (FOS) from sucrose involves fructosyltransferases (FFTs) and invertases, both of which catalyze forward (transferase) and reverse (hydrolysis) reactions. FOS yields can therefore be increased by favoring the forward reaction. We investiga...

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Foilsithe in:Front Bioeng Biotechnol
Main Authors: Burghardt, Jan Philipp, Fan, Rong, Baas, Markus, Eckhardt, Dustin, Gerlach, Doreen, Czermak, Peter
Formáid: Artigo
Teanga:Inglês
Foilsithe: Frontiers Media S.A. 2020
Ábhair:
Bioengineering and Biotechnology
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7724039/
https://ncbi.nlm.nih.gov/pubmed/33324627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.607507
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