Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing

The enzymatic production of prebiotic fructo-oligosaccharides (FOS) from sucrose involves fructosyltransferases (FFTs) and invertases, both of which catalyze forward (transferase) and reverse (hydrolysis) reactions. FOS yields can therefore be increased by favoring the forward reaction. We investiga...

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Detalles Bibliográficos
Publicado en:Front Bioeng Biotechnol
Main Authors: Burghardt, Jan Philipp, Fan, Rong, Baas, Markus, Eckhardt, Dustin, Gerlach, Doreen, Czermak, Peter
Formato: Artigo
Idioma:Inglês
Publicado: Frontiers Media S.A. 2020
Assuntos:
Bioengineering and Biotechnology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7724039/
https://ncbi.nlm.nih.gov/pubmed/33324627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.607507
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