Isolation and detection of a KDEL-tagged recombinant cholera toxin B subunit from Nicotiana benthamiana

Here we describe refined methods for the isolation and detection of a KDEL-tagged, plant-produced recombinant cholera toxin B subunit (CTB) that exhibits unique mucosal wound healing activity. The protein was transiently overexpressed in Nicotiana benthamiana, which generates some C-terminal KDEL tr...

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Bibliographic Details
Published in:Process Biochem
Main Authors: Morris, David A., Reeves, Micaela A., Royal, Joshua M., Hamorsky, Krystal T., Matoba, Nobuyuki
Format: Artigo
Language:Inglês
Published: 2020
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7723343/
https://ncbi.nlm.nih.gov/pubmed/33304198
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.procbio.2020.10.018
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