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Isolation and detection of a KDEL-tagged recombinant cholera toxin B subunit from Nicotiana benthamiana
Here we describe refined methods for the isolation and detection of a KDEL-tagged, plant-produced recombinant cholera toxin B subunit (CTB) that exhibits unique mucosal wound healing activity. The protein was transiently overexpressed in Nicotiana benthamiana, which generates some C-terminal KDEL tr...
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| Udgivet i: | Process Biochem |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7723343/ https://ncbi.nlm.nih.gov/pubmed/33304198 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.procbio.2020.10.018 |
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