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A proteolytic method for evaluating O-GlcNAcylation on proteins of similar molecular weight to antibody heavy chain after immunoprecipitation

Investigating a protein of interest that runs at the same molecular weight as antibody heavy chain is a frequent deterrent to its evaluation by immunoprecipitation. Methods of minimizing the detection of the immunoprecipitating antibody are available. However, these still present a barrier to evalua...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Anal Biochem
Hauptverfasser: Machacek, Miranda, Fields, Patrick E., Slawson, Chad
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7718295/
https://ncbi.nlm.nih.gov/pubmed/33129762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2020.114001
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