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A proteolytic method for evaluating O-GlcNAcylation on proteins of similar molecular weight to antibody heavy chain after immunoprecipitation

Investigating a protein of interest that runs at the same molecular weight as antibody heavy chain is a frequent deterrent to its evaluation by immunoprecipitation. Methods of minimizing the detection of the immunoprecipitating antibody are available. However, these still present a barrier to evalua...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Machacek, Miranda, Fields, Patrick E., Slawson, Chad
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7718295/
https://ncbi.nlm.nih.gov/pubmed/33129762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2020.114001
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