DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencing

DNA aptamers generated by cell-SELEX against bacterial cells have gained increased interest as novel and cost-effective affinity reagents for cell labelling, imaging and biosensing. Here we describe the selection and identification of DNA aptamers for bacterial cells using a combined approach based...

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Pubblicato in:Sci Rep
Autori principali: Kolm, Claudia, Cervenka, Isabella, Aschl, Ulrich J., Baumann, Niklas, Jakwerth, Stefan, Krska, Rudolf, Mach, Robert L., Sommer, Regina, DeRosa, Maria C., Kirschner, Alexander K. T., Farnleitner, Andreas H., Reischer, Georg H.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2020
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708460/
https://ncbi.nlm.nih.gov/pubmed/33262379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-77221-9
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