Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids

The extraction of nucleic acids from microorganisms for subsequent molecular diagnostic applications is still a tedious and time-consuming procedure. We developed a method for the rapid preparation of genomic DNA from bacteria based on hydrophilic ionic liquids (ILs). First, we tested eight ILs in d...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Martzy, Roland, Bica-Schröder, Katharina, Pálvölgyi, Ádám Márk, Kolm, Claudia, Jakwerth, Stefan, Kirschner, Alexander K. T., Sommer, Regina, Krska, Rudolf, Mach, Robert L., Farnleitner, Andreas H., Reischer, Georg H.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6768989/
https://ncbi.nlm.nih.gov/pubmed/31570727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-50246-5
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