Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

BACKGROUND: One ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target activity is challenging. Here, we present SMRT-OTS and Nano-OTS, two novel, amplification-free, long-re...

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Publicat a:Genome Biol
Autors principals: Höijer, Ida, Johansson, Josefin, Gudmundsson, Sanna, Chin, Chen-Shan, Bunikis, Ignas, Häggqvist, Susana, Emmanouilidou, Anastasia, Wilbe, Maria, den Hoed, Marcel, Bondeson, Marie-Louise, Feuk, Lars, Gyllensten, Ulf, Ameur, Adam
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2020
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7706270/
https://ncbi.nlm.nih.gov/pubmed/33261648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02206-w
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