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Multiple imputation and direct estimation for qPCR data with non-detects

BACKGROUND: Quantitative real-time PCR (qPCR) is one of the most widely used methods to measure gene expression. An important aspect of qPCR data that has been largely ignored is the presence of non-detects: reactions failing to exceed the quantification threshold and therefore lacking a measurement...

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Detalhes bibliográficos
Publicado no:BMC Bioinformatics
Main Authors: Sherina, Valeriia, McMurray, Helene R., Powers, Winslow, Land, Harmut, Love, Tanzy M. T., McCall, Matthew N.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7693525/
https://ncbi.nlm.nih.gov/pubmed/33243147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-020-03807-9
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