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Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!
BACKGROUND: A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in a dilution series. This method suffers from a serious prob...
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| Publicado no: | BMC Bioinformatics |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7346608/ https://ncbi.nlm.nih.gov/pubmed/32640980 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-020-03604-4 |
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