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Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!

BACKGROUND: A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in a dilution series. This method suffers from a serious prob...

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書目詳細資料
發表在:BMC Bioinformatics
主要作者: Tellinghuisen, Joel
格式: Artigo
語言:Inglês
出版: BioMed Central 2020
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7346608/
https://ncbi.nlm.nih.gov/pubmed/32640980
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-020-03604-4
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