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Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization
Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head‐to‐tail backbone cyclization of SaSr...
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| 出版年: | Chemistry |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
John Wiley and Sons Inc.
2020
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7693181/ https://ncbi.nlm.nih.gov/pubmed/32649777 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202002740 |
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