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Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization
Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head‐to‐tail backbone cyclization of SaSr...
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| Veröffentlicht in: | Chemistry |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley and Sons Inc.
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7693181/ https://ncbi.nlm.nih.gov/pubmed/32649777 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202002740 |
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