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Engineering the specificity of Streptococcus pyogenes sortase A by loop grafting

Sortases are a group of enzymes displayed on the cell‐wall of Gram‐positive bacteria. They are responsible for the attachment of virulence factors onto the peptidoglycan in a transpeptidation reaction through recognition of a pentapeptide substrate. Most housekeeping sortases recognize one specific...

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Bibliografische gegevens
Gepubliceerd in:Proteins
Hoofdauteurs: Wójcik, Magdalena, Szala, Kamil, van Merkerk, Ronald, Quax, Wim J., Boersma, Ykelien L.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: John Wiley & Sons, Inc. 2020
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7586933/
https://ncbi.nlm.nih.gov/pubmed/32501594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.25958
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