Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head‐to‐tail backbone cyclization of SaSr...

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Bibliographic Details
Published in:Chemistry
Main Authors: Zou, Zhi, Mate, Diana M., Nöth, Maximilian, Jakob, Felix, Schwaneberg, Ulrich
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2020
Subjects:
Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7693181/
https://ncbi.nlm.nih.gov/pubmed/32649777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202002740
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