Single-cell analysis of autophagy activity in normal and de novo transformed human mammary cells

Assessment of autophagy activity has historically been limited to investigations of fixed tissue or bulk cell populations. To address questions of heterogeneity and relate measurements to functional properties of viable cells isolated from primary tissue, we created a lentiviral (RFP-GFP-MAP1LC3B) v...

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Pubblicato in:Sci Rep
Autori principali: Lefort, Sylvain, Balani, Sneha, Pellacani, Davide, Guyot, Boris, Gorski, Sharon M., Maguer-Satta, Véronique, Eaves, Connie J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2020
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7679376/
https://ncbi.nlm.nih.gov/pubmed/33219251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-77347-w
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