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Single-cell analysis of autophagy activity in normal and de novo transformed human mammary cells

Assessment of autophagy activity has historically been limited to investigations of fixed tissue or bulk cell populations. To address questions of heterogeneity and relate measurements to functional properties of viable cells isolated from primary tissue, we created a lentiviral (RFP-GFP-MAP1LC3B) v...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Lefort, Sylvain, Balani, Sneha, Pellacani, Davide, Guyot, Boris, Gorski, Sharon M., Maguer-Satta, Véronique, Eaves, Connie J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7679376/
https://ncbi.nlm.nih.gov/pubmed/33219251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-77347-w
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