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RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish

We recently developed a piggyback knockdown method that was used to knockdown genes in adult zebrafish. In this method, a vivo morpholino (VMO) piggybacks an antisense deoxyoligonucleotide (dO) into the somatic cells and reduces the cognate mRNA levels. In this paper, we tested whether we can piggyb...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Raman, Revathi, Ryon, Mia, Jagadeeswaran, Pudur
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7677540/
https://ncbi.nlm.nih.gov/pubmed/33214638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-76655-5
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