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Single-pass transcription by T7 RNA polymerase

RNA molecules can be conveniently synthesized in vitro by the T7 RNA polymerase (T7 RNAP). In some experiments, such as cotranscriptional biochemical analyses, continuous synthesis of RNA is not desired. Here, we propose a method for a single-pass transcription that yields a single transcript per te...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Passalacqua, Luiz F.M., Dingilian, Armine I., Lupták, Andrej
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7668259/
https://ncbi.nlm.nih.gov/pubmed/32958559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.076778.120
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