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Single-pass transcription by T7 RNA polymerase
RNA molecules can be conveniently synthesized in vitro by the T7 RNA polymerase (T7 RNAP). In some experiments, such as cotranscriptional biochemical analyses, continuous synthesis of RNA is not desired. Here, we propose a method for a single-pass transcription that yields a single transcript per te...
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| Veröffentlicht in: | RNA |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Cold Spring Harbor Laboratory Press
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7668259/ https://ncbi.nlm.nih.gov/pubmed/32958559 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.076778.120 |
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