Estimates of Rel(Seq), Mesh1, and SAH(Mex) Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays

The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn(2+) dependent reaction. Here, thin layer chromatography (TLC) qualitative evidence is presented indicating the subst...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Potrykus, Katarzyna, Thomas, Nathan E., Bruhn-Olszewska, Bożena, Sobala, Michał, Dylewski, Maciej, James, Tamara, Cashel, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7644958/
https://ncbi.nlm.nih.gov/pubmed/33193211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2020.581271
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