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Estimates of Rel(Seq), Mesh1, and SAH(Mex) Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays
The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn(2+) dependent reaction. Here, thin layer chromatography (TLC) qualitative evidence is presented indicating the subst...
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| 發表在: | Front Microbiol |
|---|---|
| Main Authors: | , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Frontiers Media S.A.
2020
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7644958/ https://ncbi.nlm.nih.gov/pubmed/33193211 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2020.581271 |
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