Enhancing the Incorporation of Lysine Derivatives into Proteins with Methylester Forms of Unnatural Amino Acids

We have improved the incorporation of L- and D-forms of unnatural amino acid (UAA) N(ε)-thiaprolyl-L-lysine (ThzK) into ubiquitin (UB) and green fluorescent protein (GFP) by 2–6 folds with the use of the methylester forms of the UAAs in E coli cell culture. We also improved the yields of UAA-incorpo...

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Veröffentlicht in:Bioorg Med Chem Lett
Hauptverfasser: Zhou, Han, Cheung, Jenny W., Carpenter, Tomaya, Jones, Stacey, Luong, Nhu H., Tran, Nhi C., Jacobs, Savannah E., Galbada Liyanage, Sahan A., Cropp, T. Ashton, Yin, Jun
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7644286/
https://ncbi.nlm.nih.gov/pubmed/31836447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2019.126876
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