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Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast
Unnatural amino acids can be genetically incorporated into proteins in live cells by using an orthogonal tRNA/aminoacyl-tRNA synthetase pair. Here we describe a method to efficiently express the orthogonal tRNA and synthetase in Saccharomyces cerevisiae, which enables unnatural amino acids to be gen...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3965369/ https://ncbi.nlm.nih.gov/pubmed/21956564 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-331-8_12 |
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