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Fluorescent Bioorthogonal Labeling of Class B GPCRs in Live Cells
In this Method paper, we describe the protocols for selective labeling of GCGR, a member of the class B GPCR family regulating glucose homeostasis, in live cells. A two-step procedure is presented in which a strained alkene chemical reporter is inserted into any desired location within the GPCR in t...
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| 出版年: | Methods Enzymol |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2020
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7643056/ https://ncbi.nlm.nih.gov/pubmed/32713539 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2020.04.038 |
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