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Fluorescent Bioorthogonal Labeling of Class B GPCRs in Live Cells

In this Method paper, we describe the protocols for selective labeling of GCGR, a member of the class B GPCR family regulating glucose homeostasis, in live cells. A two-step procedure is presented in which a strained alkene chemical reporter is inserted into any desired location within the GPCR in t...

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Bibliografiske detaljer
Udgivet i:Methods Enzymol
Main Authors: Gangam, Srikanth Kumar, Lin, Qing
Format: Artigo
Sprog:Inglês
Udgivet: 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7643056/
https://ncbi.nlm.nih.gov/pubmed/32713539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2020.04.038
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