Multi-Scale 3D Cryo-Correlative Microscopy for Vitrified Cells

Three-dimensional (3D) visualization of vitrified cells can uncover structures of subcellular complexes without chemical fixation or staining. Here, we present a pipeline integrating three imaging modalities to visualize the same specimen at cryogenic temperature at different scales: cryo-fluorescen...

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Udgivet i:Structure
Main Authors: Wu, Gong-Her, Mitchell, Patrick G., Galaz-Montoya, Jesus G., Hecksel, Corey W., Sontag, Emily M., Gangadharan, Vimal, Marshman, Jeffrey, Mankus, David, Bisher, Margaret E., Lytton-Jean, Abigail K. R., Frydman, Judith, Czymmek, Kirk, Chiu, Wah
Format: Artigo
Sprog:Inglês
Udgivet: 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7642057/
https://ncbi.nlm.nih.gov/pubmed/32814034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2020.07.017
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