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Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components

Electron cryo-microscopy has become a routine technique to determine structure of biochemically purified herpes simplex virus capsid particles. This chapter describes the procedures of specimen preparation by cryopreservation; low dose and low temperature imaging in an electron cryo-microscope; and...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Rochat, Ryan H., Hecksel, Corey W., Chiu, Wah
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4370171/
https://ncbi.nlm.nih.gov/pubmed/24671690
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-0428-0_18
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