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Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components
Electron cryo-microscopy has become a routine technique to determine structure of biochemically purified herpes simplex virus capsid particles. This chapter describes the procedures of specimen preparation by cryopreservation; low dose and low temperature imaging in an electron cryo-microscope; and...
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4370171/ https://ncbi.nlm.nih.gov/pubmed/24671690 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-0428-0_18 |
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