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RNA recording in single bacterial cells using reprogrammed tracrRNAs

Capturing an individual cell’s transcriptional history is a challenge exacerbated by the functional heterogeneity of cellular communities. Here, we leverage reprogrammed tracrRNAs (Rptrs) to record selected cellular transcripts as stored DNA edits in single living bacterial cells. Rptrs are designed...

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Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Jiao, Chunlei, Reckstadt, Claas, König, Fabian, Homberger, Christina, Yu, Jiaqi, Vogel, Jörg, Westermann, Alexander J., Sharma, Cynthia M., Beisel, Chase L.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group US 2023
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7614944/
https://ncbi.nlm.nih.gov/pubmed/36604543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-022-01604-8
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