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Engineering an Efficient and Enantioselective Enzyme for the Morita-Baylis-Hillman Reaction

The combination of computational design and directed evolution could offer a general strategy to create enzymes with new functions. To date, this approach has delivered enzymes for a handful of model reactions. Here we show that new catalytic mechanisms can be engineered into proteins to accelerate...

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Библиографические подробности
Опубликовано в: :Nat Chem
Главные авторы: Crawshaw, Rebecca, Crossley, Amy E., Johannissen, Linus, Burke, Ashleigh J., Hay, Sam, Levy, Colin, Baker, David, Lovelock, Sarah L., Green, Anthony P.
Формат: Artigo
Язык:Inglês
Опубликовано: 2022
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7612480/
https://ncbi.nlm.nih.gov/pubmed/34916595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41557-021-00833-9
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