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Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
Structure determination by cryo-EM has rapidly grown in the last decade, however sample preparation remains a significant bottleneck. Macromolecular samples are ideally imaged directly from random orientations in a thin layer of vitreous ice. Many samples are refractory to this, however, and protein...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7612214/ https://ncbi.nlm.nih.gov/pubmed/33900294 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/62321 |
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