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Direct transfer of electron microscopy samples to wetted carbon and graphene films via a support floatation block

Support films are commonly used during cryo-EM specimen preparation to both immobilise the sample and minimise the exposure of particles at the air-water interface. Here we report preparation protocols for carbon and graphene supported single particle electron microscopy samples using a novel 3D-pri...

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Veröffentlicht in:J Struct Biol
Hauptverfasser: de Martín Garrido, Natàlia, Fu, Wencheng, Ramlaul, Kailash, Zhu, Zining, Miller, David, Boehringer, Daniel, Aylett, Christopher H.S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Academic Press 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7998342/
https://ncbi.nlm.nih.gov/pubmed/33307178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2020.107677
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