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Thermodynamic analysis of cooperative ligand binding by the ATP-binding DNA aptamer indicates a population-shift binding mechanism

The ATP-binding DNA aptamer is often used as a model system for developing new aptamer-based biosensor methods. This aptamer follows a structure-switching binding mechanism and is unusual in that it binds two copies of its ligand. We have used isothermal titration calorimetry methods to study the bi...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Slavkovic, Sladjana, Zhu, Yanrui, Churcher, Zachary R., Shoara, Aron A., Johnson, Anne E., Johnson, Philip E.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7609719/
https://ncbi.nlm.nih.gov/pubmed/33144644
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-76002-8
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