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Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells
Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex is prone to detection by DNA mismatch repair...
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| Publicado no: | PLoS Genet |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7595315/ https://ncbi.nlm.nih.gov/pubmed/33119594 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1009041 |
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