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Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells

Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex is prone to detection by DNA mismatch repair...

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Foilsithe in:PLoS Genet
Main Authors: van Ravesteyn, Thomas W., Arranz Dols, Marcos, Pieters, Wietske, Dekker, Marleen, te Riele, Hein
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2020
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7595315/
https://ncbi.nlm.nih.gov/pubmed/33119594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1009041
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