LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells

Synthetic single-stranded DNA oligonucleotides (ssODNs) can be used to generate subtle genetic modifications in eukaryotic and prokaryotic cells without the requirement for prior generation of DNA double-stranded breaks. However, DNA mismatch repair (MMR) suppresses the efficiency of gene modificati...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: van Ravesteyn, Thomas W., Dekker, Marleen, Fish, Alexander, Sixma, Titia K., Wolters, Astrid, Dekker, Rob J., te Riele, Hein P. J.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2016
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4839440/
https://ncbi.nlm.nih.gov/pubmed/26951689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1513315113
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